Figure 3
From: No evidence of XMRV in prostate cancer cohorts in the Midwestern United States
Detection of XMRV in prostate cancer tissues. (A) Specificity of anti-XMRV antiserum and anti-MLV antibody. 293T cells transfected with XMRV infectious plasmid (pcDNA3.1(-)/VP62) were stained with either rabbit anti-XMRV or goat anti-MLV. No positive staining was observed in control uninfected 293T cells. (B) Serial tissue sections from qPCR positive samples, including #51 (Gleason score (GS) 8) and #103 (GS 6) were immunostained with either anti-XMRV or anti-MLV antibody. H&E staining from each sample is also shown. (C) Serial tissue sections from qPCR positive (#112, GS 6) and negative (#47, GS 8) samples were immunostained with anti-MLV antibody, followed by TexasRed-conjugated donkey anti-rabbit antibody (Jackson ImmunoResearch Laboratories, Inc., 1:200).