HIC inhibits HIV-1 Rev function in Jurkat and CEM but not in U937 or THP-1. Jurkat, CEM, U937 and THP-1 cells were transfected with 1 μg of pDM128-RRE combined with 0.5 μg of HA-Rev and 4 μg of FLAG HIC or parent plasmid. Relative CAT activity was analysed 24 hours post-transfection as described before. Values are mean ± standard deviation. Data are representative of a minimum of three independent experiments performed in triplicate.