Parameter | Assay | NHS + C' | Day 44 serum + C' | Fold enhancement |
|---|
% infected cells | Intracellular p24 stain | 0.20 | 46.1 | 236 |
% viable cells | MTT | 101 | 20 | N/A |
RT production (pg/ml) | RT ELISA | 222 | 20495 | 92 |
- To further characterise the enhancement and elucidate whether the flow cytometry results obtained were representative of productive infection, reverse transcriptase (RT) output (to quantitate virus production) and percentage (%) cell death (normalised to uninfected control cultures) were measured by RT ELISA and MTT assay, respectively. Results shown are from enhanced and control infection of MM24.26 virus. Experiments were carried out in the presence of C', with fold enhancement calculated by dividing infection levels in the presence of patient serum (MM24 day 44) by infection in the presence of control serum (NHS).
