Figure 2

High-level C'-ADE of early viruses by autologous sequential sera. Serum sets from 10 patients were tested against autologous virus for C'-ADE activity. Sera and virus time points are shown as days following onset of symptoms characteristic of primary HIV infection (DFOS). Arrows indicate the time point from which virus was isolated. White squares represent assays conducted in the presence of HIC'; grey squares, C'. Error bars represent standard deviations from 3 experiments. To show clearly events pre-day 100 (during which the antibody response is developing rapidly and sampling is more frequent), breaks have been inserted into the X-axes. Post day-100 intervals = 100 days. Shading indicates the first appearance and continued detection of an autologous neutralising antibody response (as determined by >90% neutralisation in an independent neutralisation assay). The horizontal dashed blue line indicates the cut-off point for positive C'-ADE. Note that the scales of the Y-axes differ between subjects. The datum point equating to the enhancement depicted in Figure 1 B, C and D (MM32.10 virus with day 15 autologous serum) is circled in red.