Figure 5
From: Mutation of a diacidic motif in SIV-PBj Nef impairs T-cell activation and enteropathic disease
Kinetics of plasma viremia and lymphopenia and seroconversion in macaques inoculated intravenously with PBj-wt or PBj-Nef202/203GG. After inoculation of pig-tailed macaques with PBj-wt or PBj-Nef202/203GG virus blood samples were taken at different time-points p.i. and analyzed for cell associated viral load and relative lymphocyte counts. Data for animals #260 and #277, inoculated with the 10-fold virus dose, are shown in grey. (A and B) Cell associated viral load in the peripheral blood, determined by limiting dilution titration of PBMCs of infected macaques on C8166 cells, presented as TCID50 for animals inoculated with (A) PBj-wt virus or (B) PBj-Nef202/203GG. (C and D) Plasma viral load determined by quantitative RT-PCR on plasma of infected macaques, presented as genome copies / ml plasma for animals inoculated with (C) PBj-wt virus or (D) PBj-Nef202/203GG. (E and F) Total lymphocyte counts of infected macaques, related to preinoculation values. (G) Seroconversion of infected animals, as determined by crossreactive anti-HIV ELISA and shown as sample to cut-off value (S/CO). CO is indicated by dotted line.