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Figure 7 | Retrovirology

Figure 7

From: Mutations affecting interaction of integrase with TNPO3 do not prevent HIV-1 cDNA nuclear import

Figure 7

IN mutants are not significantly affected at the nuclear import step. SupT1 TL34 cells depleted for LEDGF/p75 were infected in a single round experiment with equal amounts of WT or mutants Bru viruses. Cellular DNA was extracted and quantified for (A) late reverse transcribed (LRT) viral cDNA products at 7 h p.i., (B) 2-LTR circles at 24 h p.i., using a real time quantitative PCR. (C) 2-LTR circles were normalized to LRT viral cDNA products. Error bars represent standard deviations of n = 2 experiments performed in duplicate. Results are expressed as the percentage of WT products.

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