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Figure 6 | Retrovirology

Figure 6

From: Mutations affecting interaction of integrase with TNPO3 do not prevent HIV-1 cDNA nuclear import

Figure 6

IN mutants are primarily impaired at the integration step. SupT1 cells were infected in a single round experiment with equal amounts of WT or mutants Bru viruses. At 7 h, 24 h and 48 h post infection (p.i.), cellular DNA was extracted and quantified for (A) late reverse transcribed (LRT) viral cDNA products at 7 h p.i., (B) 2-LTR circles at 24 h p.i., and (D) integrated proviral DNA at 48 h p.i., using a real time quantitative PCR. Results are expressed as the percentage of WT products. Depending on the mutant, error bars represent standard deviations of n = 2 to n = 6 experiments performed in duplicate. (C) 2-LTR circles were normalized to LRT viral cDNA products.

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