Conserved functions of CMV-specific CD8+ T-cells in AC#287. (A) For antigen-specific T-cell proliferation, PBMCs from #287 were cultured for 13 days with or without 100 nM CMV peptide. The number indicates the percentage of CMV tetramer+ cells in CD8+ T-cells. (B-D) PBMCs were stimulated with or without 10 μM CMV peptide for 6 hrs. IFN-γ production (B), CD69 (C) and CD107a (D) expression of CMVpp65-specific CD8+ T-cells in #287 was analyzed by flow cytometry. (B, D) The number represents the percentage of the indicated marker-positive cells in CMVpp65-specific CD8+ T-cells. (C) Bar indicates the percentage of CD69+ cells in CMV-specific CD8+ T-cells.