Figure 7From: The membrane-spanning domain of gp41 plays a critical role in intracellular trafficking of the HIV envelope proteinThe analysis of glycosylation of WT and mutant Env. The FLAG-tagged Env purified from transfected COS-7 cells was treated with Endo H or PNGase F glycosidase. The treated protein was separated by SDS-PAGE and detected by immunoblotting analysis using anti-FLAG antibody. The asterisk shows the endo H-resistant fraction of Env.Back to article page