Figure 5From: The membrane-spanning domain of gp41 plays a critical role in intracellular trafficking of the HIV envelope proteinThe transport defect of alanine insertion mutant Env. Endoplasmic reticulum (ER) (A and B) and Golgi (C to F) regions were visualized by fluorescence protein-conjugated ER or Golgi marker proteins (shown in green). FLAG tagged WT (A, C and E) and 695+2A Env (B, D and F) were stained by anti-FLAG antibody and Alexa Fluor (shown in red). The close-up of the Golgi area was shown in E and F. Nuclei of cells were stained with Hoechst 33258 (shown in blue).Back to article page