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Figure 5 | Retrovirology

Figure 5

From: The membrane-spanning domain of gp41 plays a critical role in intracellular trafficking of the HIV envelope protein

Figure 5

The transport defect of alanine insertion mutant Env. Endoplasmic reticulum (ER) (A and B) and Golgi (C to F) regions were visualized by fluorescence protein-conjugated ER or Golgi marker proteins (shown in green). FLAG tagged WT (A, C and E) and 695+2A Env (B, D and F) were stained by anti-FLAG antibody and Alexa Fluor (shown in red). The close-up of the Golgi area was shown in E and F. Nuclei of cells were stained with Hoechst 33258 (shown in blue).

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