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Figure 3 | Retrovirology

Figure 3

From: The membrane-spanning domain of gp41 plays a critical role in intracellular trafficking of the HIV envelope protein

Figure 3

The fusion activity of Ala-insertion mutants in the cell-cell fusion assay. COS-7 cells transfected with the T7 RNA polymerase expression vector and the Env expression vector were co-cultured with 293CD4 cells transfected with a plasmid containing T7 promoter-driven renilla luciferase reporter. After a 24-hr co-culture, the renilla luciferase reporter activity was measured and normalized to the firefly activities as described previously [18]. The normalized renilla luciferase activities for (A) single Ala-inserted mutant of Env, (B) the mutant Env with multiple Ala insertion, (C) mutant Env with two alanine residues inserted at positions 695 and 696 are shown. Data are the average of three independent experiments. The error bar indicates a standard error.

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