Misfolding of CasBrE SU is reversed by interactions with 4070A Env: implications for gammaretroviral neuropathogenesis

Table 1 CasBrE Env-NSC mediated superinfection of amphotropic-restricted targets through cell-cell contact

NSCs	Infectious Centers^a		Plating Efficiency^b	Infectious Center Efficiency^c
	Dunni	DunniAm		Dunni	DunniAm
hrGFP+4070A	10.5 ± 1.3	< 0.05*	2.5 ± 1	420%	< 2%
CasES+4070A	67 ± 8.3	18 ± 3	16.5 ± 3	406%	109%
CasE+4070A	49 ± 5.0	64.7 ± 7.2	16.2 ± 3.8	302%	400%

a- Mitomycin C-treated 4070A-NSCs expressing GFP, CasE or CasES were counted and serial dilutions were seeded with 10⁵ dunni fibroblasts. The number of foci expressing the viral vector once the Dunni cells reached confluence was evaluated and expressed as foci/per 100 NSCs seeded.
b- To determine the % plating efficiency, the mitomycin C-treated NSCs were counted and seeded as in "a" in the absence of dunni cells and the percentage of vector expressing cells present on these plates, versus the number seeded, was assessed at 48 hours.
c- IC efficiency is simply the number of foci/100 NSCs seeded divided by the plating efficiency expressed as a percentage.
*-The detection of an infectious center is limited by the maximum number of NSCs seeded per plate of targets. For these experiments it was limited to ≤ 2000 NSCs/plate.

ISSN: 1742-4690