Figure 7

Target cells are dually infected by 4070A and pseudotyped viral vectors. Infectious centers (ICs) were generated by seeding 4070A-infected CasE- and CasES-NSCs with target dunni fibroblasts followed by double immunostaining for CasBrE Env (697; red) to indicate vector infection and Gag (αGag, green) to depict 4070A virus infection. Abundant colocalization in the target fibroblasts is shown in the merged images (Merge) indicating that both the viral vector and 4070A genes were efficiently delivered to target cells. Bar = 100 μm.