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Table 2 Intra- and inter-assay reproducibility of BLV-CoCoMo-qPCR

From: BLV-CoCoMo-qPCR: Quantitation of bovine leukemia virus proviral load using the CoCoMo algorithm

No.

Proviral load1

Intra-assay2

Inter-assay3

 

Exp.1

Exp 2

Exp 3

Exp.1

Exp.2

Exp.3

Exp.1~3

Ns105

1998 ± 385

2107 ± 296

1581 ± 150

17.9

14.1

9.5

14.6

Ns209

3951 ± 691

3751 ± 529

3049 ± 150

17.5

14.1

4.9

13.2

Ns126

20388 ± 222

23484 ± 1854

28375 ± 1381

1.1

7.9

4.9

16.7

Ns226

30155 ± 6184

27247 ± 1454

34954 ± 3021

20.5

5.3

8.6

12.6

Ns120

57236 ± 6127

59375 ± 3195

53225 ± 2514

10.7

5.4

4.7

5.5

Ns107

90947 ± 0

73002 ± 3228

61388 ± 4779

0.0

4.4

7.8

19.8

Ns112

87377 ± 7434

94934 ± 5891

84667 ±5763

8.5

6.2

6.8

6.0

  1. 1Values represent the mean ± standard deviation (SD) of BLV proviral copy numbers in 105 cells from triplicate PCR amplifications from each sample.
  2. 2Intra-CV: Coefficient of variation between each sample.
  3. 3Inter-CV: Coefficient of variation between each experiment.