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Figure 3 | Retrovirology

Figure 3

From: GPI-anchored single chain Fv - an effective way to capture transiently-exposed neutralization epitopes on HIV-1 envelope spike

Figure 3

Effect of secretory and GPI scFvs (AB31, AB32, TG15, 4E10, 48d, X5 and AB65) on infection of HIV-1 viruses and pseudotypes. A.Effect of GPI-scFvs on transduction efficiency of HIV-1 and 10A1 pseudotypes into GPI-scFv-transduced TZM.bl. w/o: parental TZM.bl cells; *mean and standard deviation of relative luciferase activity. B.Effect of secretory scFvs on transduction efficiency of HIV-1 and 10A1 pseudotypes into secretory scFv-transduced TZM.bl. w/o: parental TZM.bl cells. C.Effect of GPI-scFvs on wild type HIV-1 infection in GPI-scFv-transduced TZM.bl. w/o: parental TZM.bl cells. D.Effect of secretory scFvs on wild type HIV-1 infection in secretory scFv-transduced TZM.bl. w/o: parental TZM.bl cells. Blue color-coated: > or = 50% inhibition; Orange color-coated: > or = 90% inhibition; Red color-coated: >or = 99% inhibition. The percentage of inhibition was based on the following calculation: (RLA in virus alone to a given transduced cell - RLA in no virus to the same transduced cell)/(RLA in virus alone to parental cells - RLA in no virus to parental cell).

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