Expression of secretory and GPI-anchored scFvs in transduced TZM.bl cells. A. Schematic diagram of the lentiviral vectors pRRL-scFv/hinge/his-tag/DAF and pRRL-scFv/hinge/his-tag. Single chain Fvs (scFvs) were derived from seven human monoclonal antibodies AB31, AB32, TG15, 48d, X5 and AB65; hinge: a human IgG3 hinge region; his-tag: a 6 histidine residue tag; DAF: the C-terminal 34 amino acid residues of decay accelerating factor. B. Western blot analysis of expression of scFvs (AB31, AB32, TG15, 48d, X5 and AB65) in TZM.bl cells transduced with lentiviral vectors pRRL-scFv/hinge/his-tag/DAF and pRRL-scFv/hinge/his-tag. GPI-scFv: GPI-anchored scFv; Sec-scFv: secretory scFv; anti-his: anti-his-tag antibody. C. FACS analysis of cell surface expression of scFv/hinge/histag/DAF in mock-, scFvs (AB31, AB32, TG15, 48d, X5 and AB65)/hinge/histag/DAF- or m-scFv(TG15)-transduced TZM.bl cells with or without PI-PLC treatment.