Figure 2

Estimation of Fractional Dimerization. (A) There are three forms of Nef dimers possible- homodimers of tagged Nefs (T₂) and untagged (U₂) Nefs and heterodimers (TU, UT). We have assumed a random assortment of tagged and untagged Nefs with a binomial distribution (1:2:1) of dimeric forms. (B) The equation for the fractional dimerization (FD). (C) The corrected densities (divided by 1000), determined by ImageJ, for the experiment presented in Figure 1A, B were used to calculate FD's. (D) The corrected densities/1000 determined by ImageJ for the experiment presented in Figure 3A, B and the calculated FD's are given. (E) The corrected densities/1000 for the experiment in Figure 4A and the FD are presented.