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Figure 5 | Retrovirology

Figure 5

From: Multiple sites in the N-terminal half of simian immunodeficiency virus capsid protein contribute to evasion from rhesus monkey TRIM5α-mediated restriction

Figure 5

Activity of GH123, SIVmac239, and their derivatives to saturate TRIM5α in Rh cells. (A) Rh FRhK-4 cells were pretreated with equal amounts of VSV-G pseudotyped particles (800 ng of p25 or p27) of GH123, GH123/Q, GH123/CypS, GH123/CypS 120Q, GH/NSCG, GH/SCA N-G, GH/SCA CypG, GH/SCA, SIVmac239 or SIVmac239/P for 2 h. Cells were then infected with the VSV-G pseudotyped GFP-expressing HIV-1 vector carrying SIVmac L4/5. Data from triplicate samples (means ± SD) expressed as % GFP positive cells subtracted with the value of mock-treated cells (24.88%) are shown. Statistical significance of differences was calculated using the t-test. Asterisks above bars show differences between indicated viruses and SIVmac239. ***, P < 0.001; **, P < 0.01; ns, not significant. The statistical significance of differences between GH123 and GH123/CypS and that between GH123 and GH/SCA CypG were both < 0.001.

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Retrovirology

ISSN: 1742-4690

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