Table 1 List of the primers used in PCR for modification
Name | Primer sequence (5'-3') |
|---|---|
CN54145F | GCTCTAGAGATATCGACACCATGGACAGGGCCAAGCTGCTGCTG |
CN54145R | GTGAACAGGGTGAGGCAGGGCTACTGAGGATCCGTCGACCG |
145M1u | ACCACCGAGTTCTGCGCCAGCGACG |
145M1d | CGCAGAACTCGGTGGTGGTGGCGCCCTTCCACACGG |
145M2u | AACCAGGACACCTACCACGAGACC |
145M2d | CTCCTCGTGGTAGGTGTCCTGGTTGCTGCTCACGTTCCT |
145M3u | ACCGTGGTGGAGGACAGGAAGCAGAC |
145M3d | TTCCTGTCCTCCACCACGGTGGTGGCGTTG |
145M4u | CTACGAGAAGAACAGCCAGGAGTACTACAGGCTGATC |
145M4d | CCTGGCTGTTCTTCTCGTAGTTCTTCTTGGT |
145M5u | ATCTTCAACCGCACCCAGCCCTGCTACAACGTGAGCACCG |
145M5d | GTTGTAGCAGGGCTGGGTGCGGTTGAAGATCTTGTC |