Figure 6
Specific IFN-gamma secretion detected by ELISPOT. The T cell immunity was quantified with an IFN-γ-based ELISPOT assay with SHIVchn19 (20-mer) peptides ENV1 (4830-4871) or ENV2 pools (4872-4913). a, e & i) Elispot results in different immunized groups stimulated with stimuli as indicated on the left. b, f & k) Number of SFC in one million splenocytes stimulated by the ENV1 pool. c, g & l) Number of SFC in one million splenocytes stimulated by the ENV2 pool. d, h & m) Env-specific T cell responses against these two peptide pools were compiled together as the total T cell responses for each mouse and were graphed into groups. The mock group generated <50 SFCs/106 splenocytes. The significance of differences among the different groups was calculated using a statistical method of one-way analysis of variance (GraphPad prism4.0); SFC, spot forming cell; * p < 0.05, ** p < 0.01. The error bars indicate SEM (the standard error of the mean).