Characterization of antibodies elicited by XMRV infection and development of immunoassays useful for epidemiologic studies

Table 2 Antibody titers of predominant responses in selected bleeds of XMRV-infected macaques and goat polyclonal antibodies to MuLV.

Sample	Days post XMRV Infection			Antibody Titers by ARCHITECT CMIAs
	1^st Infection	2^nd Infection	Immuni- zation	Anti-gp70	Anti-p15E	Anti-p30
RIl-10	42			800	40	2
	134			3,200	160	10
	167	9		128,000	12,800	640
	210	52		32,000	800	80
	291	133	16	16,000	8,000	4,000
RYh-10	42			1,600	80	10
	134			1,600	80	10
	167	9		32,000	6,400	640
	210	52		8,000	400	80
	291	133	16	4,000	4,000	2,000
RLq-10	42			800	80	1
	134			800	160	1
Anti-MuLV pAb				16,000	32,000	64,000
Anti-Env pAb				10,000
Anti-His Mab				0.039 nM		1.18 nM
Anti-p30 Mab						0.56 nM

Antibody titers were determined by the three direct gp70, p15E and p30 CMIAs on samples serially diluted with negative normal human plasma, and expressed as the reciprocal of the bleed dilution giving a reactive result. For comparison, detection limits of the anti-His and anti-p30 monoclonal antibodies were also listed.

ISSN: 1742-4690