Identification of host proteins associated with HIV-1 preintegration complexes isolated from infected CD4+ cells

Retrovirology

Table 2 Host proteins selectively co-purifying with HIV-1 PICs

Host Proteins	Accession numbers	Molecular Weight	No. of peptides
Chromatin organization
Barrier-to-autointegration factor	baf_bovin	10 kDa	3
Nucleosome assembly protein 1-like 1	np1l1_bovin	45 kDa	5
Histone-binding protein RBBP4	rbbp4_bovin	48 kDa	3
*Transcription regulation*
Acidic leucine-rich nuclear phosphoprotein 32 family member A	an32a_bovin	29 kDa	4
Acidic leucine-rich nuclear phosphoprotein 32 family member E	an32e_human	31 kDa	3
Calreticulin	calr_cerae	48 kDa	5
NF-kappa-B essential modulator	nemo_bovin	49 kDa	7
Non-POU domain-containing octamer-binding protein	nono_human	54 kDa	8
RNA polymerase-associated protein LEO1	leo1_human	75 kDa	6
*RNA processing/localization*
ATP-dependent RNA helicase DDX19A	dd19a_bovin	54 kDa	4
Double-stranded RNA-binding protein Staufen homolog 1	stau1_human	63 kDa	2
Heterogeneous nuclear ribonucleoprotein H1	hnrh1_human	49 kDa	2
Heterogeneous nuclear ribonucleoprotein H3	hnrh3_human	37 kDa	3
Plasminogen activator inhibitor 1 RNA-binding protein	pairb_human	45 kDa	7
Splicing factor 3B subunit 2	sf3b2_human	98 kDa	19
Splicing factor, arginine/serine-rich 3	sfrs3_bovin	19 kDa	3
U4/U6.U5 tri-snRNP-associated protein 1	snut1_human	90 kDa	17
*Translation*
Eukaryotic translation initiation factor 4 gamma 1	if4g1_human	176 kDa	3
*Cytoplasmic trafficking*
Dynactin subunit 2	dctn2_human	44 kDa	3

The host proteins have been broadly categorized based on the known physiological function. The number of peptides identified by mass spectroscopic analysis and assigned to the specific protein in the database is shown.

ISSN: 1742-4690