Figure 3

Representative MS/MS data for HIV-1 and host proteins associated with PICs. (A) HIV-1 integrase peptide AMASDFNLPPVVAK. (B) HIV-1 Rev peptide SAEPVPLQLPPLER. (C) Cellular barrier-to-autointegration factor (BAF) peptide KDEDLFR. The 'b" and "y" ion series derived from the amide bond cleavage during collision induced dissociation of the peptide provide amino acid sequence information. The b-ion series (shown in red) is read from the N-terminus to C-terminus, while the y-ion series (shown in blue) is read from the C-terminus to N-terminus, providing thus complementary sequence information [25]. Other minor fragments resulted from peptide fragmentations at other sites are shown in green.