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Figure 7 | Retrovirology

Figure 7

From: Structural features in the Rous sarcoma virus RNA stability element are necessary for sensing the correct termination codon

Figure 7

The minimal RSE functions only after the gag termination codon. The RSE was cloned in the forward (2660-2880 For) and reverse orientation (2660-2880 Rev) after a premature termination codon within gag at position 1250. The minimal RSE (2577-2732) and a slightly longer RSE fragment (2567-2732) were cloned after the same premature termination codon. 2660-2880 For is a previously described functional fragment of the RSE (Cfor; [25]). The minimal RSE is unable to stabilize the RNA when placed after a premature termination codon within gag. RNA levels were assayed by RNase protection assay. Representative gels are shown below each graph. Stars indicate a significant reduction compared to the wild-type virus (***: p < 0.0001)

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Retrovirology

ISSN: 1742-4690

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