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Figure 5 | Retrovirology

Figure 5

From: Structural features in the Rous sarcoma virus RNA stability element are necessary for sensing the correct termination codon

Figure 5

The immediate termination codon context did not affect levels of the unspliced viral RNA. A. The fourth nucleotide of the termination codon signal was altered to each of the four possible ribonucleotides. This was done at the gag stop codon, with and without the RSE and at a premature termination codon at nucleotide 1250. Steady-state levels of RNA were assayed by RNase protection assay. These mutations did not significantly affect the stability of the wild-type viral RNA (WT gag UAG), nor the efficiency of decay of the RNAs bearing a premature termination codon within gag (PTC UAG) or lacking the RSE (ΔRSE UAG). B. The stop codon of the gag termination codon (UAG) was altered to each of the other two stop codons (UAA and UGA). The level of steady-state viral RNA was not affected, as assayed by RNase protection assay. Representative RNase protections are shown. Stars indicate a significant reduction compared to the wild-type virus (**: p < 0.001; ***: p < 0.0001)

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