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Table 2 Comparison between the predicted and the measured heterodimerization efficiencies.

From: Murine leukemia virus RNA dimerization is coupled to transcription and splicing processes

Experiment 1

SD' ORIGIN

Predicted distribution of homo- and hetero- dimers (1)

% of heterodimers captured in RCA (2)

randomness of heterodimerization

  

FLFL (%)

SD'SD' (%)

FLSD' (%)

FLSD' (%)

prediction/experiment

 

transcription in same locus as FL

24.4

25.6

50

32.7

1.53

 

transcription in distinct locus to FL

60.9

4.8

34.2

0.41

83.4

 

splicing

99.5

0.0006

0.5

0.17

2.9

Experiment 2

SD' ORIGIN

Predicted distribution of homo- and hetero- dimers (1)

% of heterodimers captured in RCA (2)

randomness of heterodimerization

  

FLFL (%)

SD'SD' (%)

FLSD' (%)

FLSD' (%)

prediction/experiment

 

transcription in same locus as FL

28.2

22

50

33.1

1.51

 

transcription in distinct locus to FL

79.7

1.2

19.2

0.38

50.53

 

splicing

99.3

0.001

0.7

0.29

2.41

  1. (1) To deduce the distribution of FLSD' RNA heterodimers predicted for random RNA dimerization, we used the Hardy-Weinberg equation (A2 + 2AB + B2 = 1), as previously described in details by Flynn et al. [29]. In this equation, A2 and B2 represent the percentage of FLFL and SD'SD' homodimers, respectively, and 2AB the FLSD' heterodimer population. Based on proportions of FL and SD' RNAs experimentally determined in virion input (Table 1), this equation allows the calculation of predicted percentages of AA (FLFL) and BB (SD'SD') homodimers in the viral population, and AB heterodimers (FLSD') represent the remaining percentage of the population. (2) The proportion of heterodimer experimentally determined by RCA was calculated from %SD' given in Table 1 as (2 × %SD'). (3) To determine the randomness of heterodimerization in the different HEK 293-derived cell-lines, the %FLSD' determined by the capture experiments were compared to that obtained by the prediction (predicted/measured).