Murine leukemia virus RNA dimerization is coupled to transcription and splicing processes

Retrovirology

Table 1 Comparative study of heterodimerization frequencies for SD' RNA produced in the different cellular contexts.

Experiment 1	SD' ORIGIN	VIRION INPUT⁽¹⁾			ELUTION⁽²⁾			%SD' ⁽⁴⁾(elution/input) × 100
		FL (cps)	SD' (cps)	%SD'	FL (cps)	SD' (cps)	%SD' ⁽³⁾
	transcription in same locus as FL	4.13E+06	4.24E+06	50.63	2.47E+05	4.83E+04	16.35	32.3
	transcription in distinct locus to FL	1.28E+08	3.60E+07	21.93	7.73E+06	1.60E+04	0.207	0.9
	splicing	9.80E+07	2.37E+05	0.24	6.34E+06	5.54E+03	0.087	36.1
Experiment 2	SD' ORIGIN	VIRION INPUT ⁽¹⁾			ELUTION ⁽²⁾			%SD' ⁽⁴⁾ (elution/input) × 100
		FL (cps)	SD' (cps)	%SD'	FL (cps)	SD' (cps)	%SD' ⁽³⁾
	transcription in same locus as FL	1.66E+07	1.47E+07	46.93	1.28E+06	2.54E+05	16.54	35.3
	transcription in distinct locus to FL	2.86E+08	3.44E+07	10.74	9.81E+06	1.86E+04	0.19	1.8
	splicing	8.57E+07	2.93E+05	0.34	6.21E+06	8.97E+03	0.144	42.4

Two independent RCA experiments were conducted from each HEK-293 cell line stably established as described in Fig.2. (1) Proportion of FL and SD' RNAs in virion input. The copies of FL and SD' RNAs determined in total virion samples before the RCA are indicated as well as the corresponding percent of SD' RNA in input. (2) The copies of captured FL and SD' RNAs quantified in total elution samples are indicated. (3) The % SD' in the elution was calculated as (SD'/(FL+SD')) × 100. (4) The FL RNA was the oligonucleotide-bound RNA, which should be retained by the beads and present in the elution. The SD' RNA was retained on the beads via its association with FL RNA and represents the heterodimer population. Based on the proportion of SD' in input, the proportion of SD' contributing to heterodimerization was calculated as the ratio of elution/input for SD' which corresponds to some extent to the heterodimerization efficiency.

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