Figure 5

ERK and PI3K pathways regulate cell migration and proliferation. A549 (A) or BEAS-2B (B) cells were allowed to migrate across the 8 μm porous membrane in a Boyden chamber either alone or in the presence of PI3K inhibitor LY294002 (20 μM) or MEK inhibitors U0126 (20 μM) or PD98059 (20 μM) in response to serum. After 15 h, the migrated cells were fixed, stained and counted. (A) *P < 0.0001; (B) *P < 0.0003). (C) A549-Env cells were allowed to proliferate either alone or in the presence of MEK inhibitor U0126 (1 μM) or (5 μM) or PI3K inhibitor LY294002 (5 μM) or (20 μM). Data shown represent proliferation at 72 h. (*P < 0.0001).