HBZ activates Dkk1 expression in an uninfected and an HTLV-1-infected T-cell line. (A) Dkk1 expression in uninfected and HTLV-1-infected T-cell lines. Levels of Dkk1 and UBE2D2 mRNA were analyzed by reverse transcriptase PCR. Cell lines and expression vectors are indicated. + RT and -RT denote cDNA synthesis with and without (mock synthesis) reverse transcriptase, respectively. (B) Ectopic expression of HBZ in transfected cells. Total cellular lysates from Jurkat (35 μg) and MT-2 (125 μg) cells were subjected to Western blot analysis using antibodies directed against Myc and β actin, as indicated.