Figure 3
PBMC gene expression patterns of HIV-1 biological clones. mRNA expression levels in PBMC's infected with the HIV-1 biological clones B1.1, B1.3, B2.5 (patient L) and B3.1, B4.2, and B4.4 (patient P), as well as uninfected PBMC's were analysed with the RT2Profiler™ PCR Array Human Inflammatory Cytokines and Receptors (SABiosciences). Cultures were infected with HIV-1 at an MOI of 0.05. After two hours, the inoculum was removed by centrifugation. Total RNA was isolated 6 hours after infection. Experiments were performed in triplicate. Expression profiles were analysed with the GCNPro™ (Gene Network Central) software [68]. Clustering of the gene expression profiles induced by the HIV-1 clones is shown for a selection of genes from a representative experiment. Green colour indicates increased mRNA expression, red colour indicates decreased mRNA expression compared to the uninfected PBMC's.