The mutation T477A in HIV-1 reverse transcriptase (RT) restores normal proteolytic processing of RT in virus with Gag-Pol mutated in the p51-RNH cleavage site

Table 1 Replication capacity of HIV-1 with mutations in the p51↓RNH cleavage site ± T477A

RT p51↓RNH mutation	Virus titer (% wild-type control)^a
	- T477A	+ T477A
WT	100	100 ± 30	(N.S.)^b
F440V	< 1 ± 1	10 ± 3	(p < 0.01)
T439S/V442G	---^c	---
Y441I/V442K	---	---
F440A	<1 ± 1	10 ± 5	(p < 0.01)
F440A/Y441A	<1 ± 1	1 ± 1	(N.S.)
F440W/Y441W	<1 ± 1	32 ± 14	(p < 0.01)
E438N	<1 ± 1	5 ± 4	(p < 0.5)

^aInfectious virus titer (TCID₅₀/ml) was determined in MT-2 cells and was normalized by dividing by the amount of viral p24 (ng/ml), as described in Materials and Methods. Data are presented as mean % wild-type virus titer ± standard deviation from four individual experiments.
^bP values were calculated using a one-tailed Student's t-test assuming equal variance. N.S., not significant.
^c No virus titer noted

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