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Table 1 Replication capacity of HIV-1 with mutations in the p51↓RNH cleavage site ± T477A

From: The mutation T477A in HIV-1 reverse transcriptase (RT) restores normal proteolytic processing of RT in virus with Gag-Pol mutated in the p51-RNH cleavage site

RT p51↓RNH mutation Virus titer (% wild-type control)a  
  - T477A + T477A  
WT 100 100 ± 30 (N.S.)b
F440V < 1 ± 1 10 ± 3 (p < 0.01)
T439S/V442G ---c ---  
Y441I/V442K --- ---  
F440A <1 ± 1 10 ± 5 (p < 0.01)
F440A/Y441A <1 ± 1 1 ± 1 (N.S.)
F440W/Y441W <1 ± 1 32 ± 14 (p < 0.01)
E438N <1 ± 1 5 ± 4 (p < 0.5)
  1. aInfectious virus titer (TCID50/ml) was determined in MT-2 cells and was normalized by dividing by the amount of viral p24 (ng/ml), as described in Materials and Methods. Data are presented as mean % wild-type virus titer ± standard deviation from four individual experiments.
  2. bP values were calculated using a one-tailed Student's t-test assuming equal variance. N.S., not significant.
  3. c No virus titer noted