Figure 3
From: Cell cycle G2/M arrest through an S phase-dependent mechanism by HIV-1 viral protein R
Chk1-Ser345is activated by Vpr and HU/UV with different cell cycle outcomes. Synchronized G1/S HeLa cells by DT were treated with HU, UV or transduced with Adv-Vpr at time 0, collected at the indicated time, and then subjected to Western blot analysis (A) using anti-Chk1-Ser345 and anti-γH2AX-Ser139 antibodies. The cell cycle profiles of differently treated cells were analyzed at the indicated time after the DT release by flow cytometric analysis (B).