Figure 6

Western blot analysis of the CA and cyclophilin A (CypA) in particles of HIV-1 derivatives. Viral particles of the indicated HIV-1 derivatives were purified by ultracentrifugation through a 20% sucrose cushion. CypA, p24, and p55 proteins were visualized by Western blotting (WB) using anti-CypA and anti-p24 antibody, respectively. "H" and "S" denote the amino acid sequences derived from HIV-1 and SIVmac239, respectively. The ratio of the amount of p24 to that of p55 of each virus is shown at the bottom. A representative of three independent experiments is shown.