Figure 5

Viral sensitivity to TRIM5α-mediated restriction in a single-round infection assay. CEMss cells were infected with SeVs expressing CM (black diamonds), human (Hu: gray circles), rhesus monkey (Rh: black triangles), or CM-SRPY(-) (white circles) TRIM5α. The cells were then superinfected with serially diluted HIV-1-GFP with the indicated CA. (B) The percentage of the GFP-positive cells in the presence of TRIM5α was divided by the percentage of GFP-positive cells in the presence of CM SPRY (-) TRIM5α to define percent infection. The differences in percent infection between WT-GFP and G116E-GFP, or 4/5S6/7S-GFP and 4/5SG116E6/7S-GFP were statistically evaluated by unpaired t test (*: P < 0.05, **; P < 0.01). The representative results of three independent experiments with similar results are shown. (C) TK-ts13 cells stably expressing CM (black diamonds), human (Hu: gray circles), or CM-SRPY(-) (white circles) TRIM5α were infected with serially diluted lentivector expressing GFP under the control of cytomegalovirus promoter with the indicated CA. (D) The percentage of the GFP-positive cells in the presence of TRIM5α was divided by the percentage of GFP-positive cells in the presence of CM SPRY (-) TRIM5α to define percent infection. The differences in percent infection between the wild type and G116E were statistically evaluated by unpaired t test (**; P < 0.01). The representative results of three independent experiments with similar results are shown.