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Figure 5 | Retrovirology

Figure 5

From: Transcriptome analysis of monocyte-HIV interactions

Figure 5

A) Levels of integrated proviral DNA in MDM and resting PBMC (pre-)treated with NAMPT (200 ng/ml) and infected with HIV BaL at 0.1 and 0.001 MOI, normalised to ERV-3, as assessed by RT-qPCR; B) viral binding to MDM, as quantified by p24 concentrations in cell lysates after 2 hours incubation and washing of the unbound virus; C) expression of CCR5 and CXCR4 on MDM treated 2 days with NAMPT (200 ng/ml) and LPS (100 ng/ml), as assessed by flow cytometry[39]; D) secretion of the β-chemokines MIP1α, MIP1β and RANTES by MDM treated 2 days with NAMPT (200 ng/ml) and LPS (100 ng/ml), as assessed by CBA. MFI: mean fluorescence intensity.

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