Figure 5

A) Levels of integrated proviral DNA in MDM and resting PBMC (pre-)treated with NAMPT (200 ng/ml) and infected with HIV _BaL at 0.1 and 0.001 MOI, normalised to ERV-3, as assessed by RT-qPCR; B) viral binding to MDM, as quantified by p24 concentrations in cell lysates after 2 hours incubation and washing of the unbound virus; C) expression of CCR5 and CXCR4 on MDM treated 2 days with NAMPT (200 ng/ml) and LPS (100 ng/ml), as assessed by flow cytometry[39]; D) secretion of the β-chemokines MIP1α, MIP1β and RANTES by MDM treated 2 days with NAMPT (200 ng/ml) and LPS (100 ng/ml), as assessed by CBA. MFI: mean fluorescence intensity.