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Figure 5 | Retrovirology

Figure 5

From: HIV-1 Vpu and HIV-2 Env counteract BST-2/tetherin by sequestration in a perinuclear compartment

Figure 5

Redistribution of EGFP-tetherin by functional anti-tetherin factors. (A) HeLa cells were co-transfected with 100 ng of EGFP-tetherin and the indicated HIV proteins. EGFP-tetherin was detected using an anti-GFP antibody, and was found to be removed from the cell surface and concentrated internally by expression of both Vpu (Vphu-HcRed) and ROD10 Env. The non-functional Env proteins from ROD14 or ROD10(Y707A) had no effect on cell surface EGFP-tetherin levels, although we frequently observed that the EGFP-tetherin signal was brighter with more visible intracellular puncta in the co-transfected cells. Scale bars represent 10 μM. (B) The degree of co-localization of EGFP-tetherin with markers for the TGN or endocytosed transferrin, in the presence of Vpu or ROD10 Env, was calculated using Pearson's coefficients. Statistical significance was calculated using unpaired t-tests, ** indicates p < 0.01 compared to control, non-transfected cells.

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