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Table 2 Kinetic parameters of the polymerization activities of SFVmac and PFV PR-RT

From: Biophysical and enzymatic properties of the simian and prototype foamy virus reverse transcriptases

enzyme KD DNA/RNA (nM) KD DNA/DNA (nM) KM 1)
(TTP/rAdT) (μM)
kcat1) (TTP/rAdT) (s-1) KM2)(dNTPs/M13) (μM) kcat2) (dNTPs/M13) (s-1)
PFV PR-RT 9.9 (± 1.6) 44.4 (± 3.0) 45 (± 12) 7.1 (± 0.9) 46 (± 9) 3 (± 0.3)
SFVmac PR-RT 32.4 (± 4.2)3) 36.4 (± 2.4)3) 40.1 (± 4.0)3) 5.5 (± 0.3)4) 45 (± 3) 4 (± 0.1)
  1. 1) KM and kcat-values, respectively, determined for TTP on the homopolymeric substrate poly(rA)/oligo(dT).
  2. 2) KM and kcat-values, respectively, determined for dNTPs on a heteropolymeric single stranded M13 substrate
  3. 3) Data adopted from [14]
  4. 4) vmax-value used for kcat calculation derived from [14].