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Table 2 Kinetic parameters of the polymerization activities of SFVmac and PFV PR-RT

From: Biophysical and enzymatic properties of the simian and prototype foamy virus reverse transcriptases

enzyme

KD DNA/RNA (nM)

KD DNA/DNA (nM)

KM 1)

(TTP/rAdT) (μM)

kcat1) (TTP/rAdT) (s-1)

KM2)(dNTPs/M13) (μM)

kcat2) (dNTPs/M13) (s-1)

PFV PR-RT

9.9 (± 1.6)

44.4 (± 3.0)

45 (± 12)

7.1 (± 0.9)

46 (± 9)

3 (± 0.3)

SFVmac PR-RT

32.4 (± 4.2)3)

36.4 (± 2.4)3)

40.1 (± 4.0)3)

5.5 (± 0.3)4)

45 (± 3)

4 (± 0.1)

  1. 1) KM and kcat-values, respectively, determined for TTP on the homopolymeric substrate poly(rA)/oligo(dT).
  2. 2) KM and kcat-values, respectively, determined for dNTPs on a heteropolymeric single stranded M13 substrate
  3. 3) Data adopted from [14]
  4. 4) vmax-value used for kcat calculation derived from [14].
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Retrovirology

ISSN: 1742-4690

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