Figure 4From: Biophysical and enzymatic properties of the simian and prototype foamy virus reverse transcriptasesDetermination of K D -values by fluorescence anisotropy measurements. 15 nM of a fluorescently labeled DNA/DNA (black circle) or DNA/RNA (black square) P/T substrate was titrated with PFV PR-RT at 25°C. The curves show the best fit to a two component binding equation [14] describing the binding equilibrium with KD-values shown in Table 2.Back to article page