Figure 3

DNA-dependent DNA polymerase activity on a heteropolymeric substrate. Reactions were carried out at 37°C for the times indicated on top with 6 nM of the M13 P/T substrate, 85 nM of PFV or SFV PR-RT and 150 μM of each dNTP, analyzed by denaturing gel electrophoresis on a 10% sequencing gel and visualized by phosphoimaging. DNA size markers are marked on the right. - RT, assay without enzyme;