Podoplanin is not expressed on HIV-infected T-cells. (A) C8166-SEAP cells were infected with an HIV-1 NL4-3 variant bearing the EGFP gene in place of nef. At 7 days post infection the infected cells were stained with podoplanin-specific antibody 18H5 and annexin V and analyzed by flow cytometry. Similar results were obtained in an independent experiment. (B) The experiment was conducted as described in (A). However, PHA stimulated PBMCs were infected and stained. The results were confirmed in two separate experiments.