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Figure 1 | Retrovirology

Figure 1

From: Incorporation of podoplanin into HIV released from HEK-293T cells, but not PBMC, is required for efficient binding to the attachment factor CLEC-2

Figure 1

CLEC-2 does not recognize the viral Env protein. (A) 293T cells were either control transfected with empty vector or transfected with an HIV-1 NL4-3 Env expression plasmid. Subsequently, the cells were preincubated with PBS or mannan and then DC-SIGN-Fc (left panel) or CLEC-2-Fc (right panel) fusion proteins or an Fc-control protein (black bars) were added. Unbound proteins were removed by washing and bound proteins detected by flow cytometry. The results represent the average of the geometric mean channel fluorescence (GMCF) measured in four independent experiments. Error bars indicate standard error of the mean (SEM). (B) 293T cells were transfected with DC-SIGN, CLEC-2 or empty vector and incubated with soluble HIV-1 Env gp120-Fc fusion protein or control Fc-protein. Unbound proteins were removed by washing and bound proteins detected by flow cytometry. The results represent the average ± SEM of the GMCF measured in three independent experiments. GMCF: geometric mean channel fluorescence, SEM: standard error of the mean.

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