Host cell characteristics of PFV Env-mediated transduction and Gag-GFP particle binding. (A, B) Evaluation of different retroviral vector titers on various adherent- (A) or suspension (B) cell lines. mock: non-infected cells; PFV: PFV vector transduced; HIV-PFV: HIV - PFV Env pseudotype transduced; HIV-VSV-G: HIV - VSV-G pseudotype transduced. (C, D) Comparison of mean EGFP fluorescence signals of various adherent- (C) or suspension (D) cell lines after incubation with different Gag-GFP-tagged retroviral particles. Mock: PBS incubated; PFV PGwt +Env: PFV Env containing C-GFP-tagged PFV Gag wt particles; PFV PGM3 ΔEnv: Env-deficient C-GFP-tagged PFV Gag PGM3 particles; PFV PGM3 +Env: Env containing C-GFP-tagged PFV Gag PGM3 particles; HIV ΔEnv: Env-deficient GFP-tagged HIV VLPs; HIV PFV: PFV Env pseudotyped HIV VLPs; HIV-VSV-G: VSV-G pseudotyped HIV VLPs.