Figure 5

Comparative analysis of Gag-GFP labelled PFV particles by CLSM and AFM. Panel A shows the CLSM image of a 100 nm fluorescent bead (on the left) and a PFV virion (on the right) supported on poly-D-lysine coated mica. The high PMT electronic gain necessary to detect the signal from the PFV virion resulted in saturation of the pixels corresponding to the fluorescent bead. Panel B shows the topographical AFM image of the same part of the sample shown in panel A.