HIV gag gene expression in non-permissive (np) and permissive (p) cells. A. Western blot analysis employing antibodies to HIV-CA and cellular tubulin of equal amounts of lysates of the indicated cell lines infected to equal levels with HIV-Wt or HIV-Env-Tr712. In this experiment, the amounts of virus released into the culture supernatants (determined by HIV-CA ELISA) were 14 ng/ml and 9 ng/ml for HIV-Wt and HIV-Env-Tr712, respectively, produced in H9 (np) cells and 43 ng/ml for both viruses produced in MT-4 (p) cells. B. Western blot analysis as in A of the indicated cell lines infected to equal levels with HIV-Wt. The positions of the detected gp120/gp160, Pr55gag, p24 and cellular tubulin proteins are given on the right.