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Figure 3 | Retrovirology

Figure 3

From: The RNA binding protein HuR does not interact directly with HIV-1 reverse transcriptase and does not affect reverse transcription in vitro

Figure 3

1H,15N-HSQC NMR analyses to probe for binding between HIV-1 RT and HuR. (A) Superimposed 1H,15N-HSQC spectra of 30 μM of the [15N]-labeled RRM 3 domain of HuR in the absence (blue) and presence (red) of 60 μM unlabeled full-length HIV-1 RT. (B) Superimposed 1H,15N-HSQC spectra of 200 μM of the [15N]-labeled RRM 1&2 domains of HuR in the absence (blue) and presence (red) of 400 μM of the unlabeled HIV-1 RT RNase H domain. (C) Superimposed 1H,15N-HSQC spectra of 60 μM of the [15N]-labeled HIV-1 RT RNaseH domain in the absence (blue) and presence (red) of 60 μM of unlabeled NusA-HuR.

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Retrovirology

ISSN: 1742-4690

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