Figure 8
From: Vpu-dependent block to incorporation of GaLV Env into lentiviral vectors
Representative Western blots of levels of FLAG-tagged GaLV Env Pr85 precursor and SU subunit in presence of pHR' and either control plasmid pGEM (Ctrl.), R8.2 or R8.2ΔVpu. Cells were treated with either the proteasomal inhibitor MG132, the lysosomal inhibitor bafilomycin A1, or DMSO. Samples were deglycosylated using endoH prior to SDS-PAGE, and analyzed by immunoblotting with anti-FLAG antibody.