Figure 5
From: Vpu-dependent block to incorporation of GaLV Env into lentiviral vectors
Representative Western blot and quantitative analysis of signal density on blots from three independent experiments analyzing GaLV TM levels in lysates of 293T cells co-transfected with GaLV Env, pHR' and the indicated packaging plasmids. (SN) indicates Vpu containing substitutions S52N and S56N. Immunological detection of HIV-1 p24 and cellular actin levels were included as controls.