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Figure 5 | Retrovirology

Figure 5

From: Modulation of the virus-receptor interaction by mutations in the V5 loop of feline immunodeficiency virus (FIV) following in vivoescape from neutralising antibody

Figure 5

A) Sensitivity of Env variants to neutralisation by homologous plasma from A613. HIV(FIV) luciferase pseudotypes bearing the B12, B14, B19, B23, B25, B28, B30, B31, B32 and GL8 Envs were incubated with 1:100, 1:1000, 1:10,000 or 1:100,000 dilutions of A613 plasma and plated onto CLL-CD134 cells[27]. Luciferase activity was measured at 72 hours post-infection. Each point represents the mean of triplicate estimations and is expressed as percent neutralisation relative to the no plasma control. Each graph is typical of at least two independent experiments. B) Neutralising activity correlates with variability in the V5 region of Env. Amino acid residues are coloured according to chemical type: basic (dark blue), acidic (red), hydrophobic (white), hydroxyl (salmon pink), sulphur-containing (yellow) or amido (lavender blue). Presence or absence of neutralisation is summarised alongside by a green tick or red cross respectively.

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