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Table 1 Comparison of titers of lentiviral vectors pseudotyped with ALV-A or ALV-B Env glycoproteins in cell lines expressing TVA and TVB receptors, EpoR or c-kit

From: Cell-specific targeting of lentiviral vectors mediated by fusion proteins derived from Sindbis virus, vesicular stomatitis virus, or avian sarcoma/leukosis virus

Vector pseudotypes

Cells

Titers (TU/ml)d

ALV-A

293 DK-7a

1.57 ± 0.47 × 108

ALV-A/Epo

293 or 293T

293T-EpoRb

<104

8.60 ± 0.70 × 107

ALV-A/SCF

MO7-ec

1.17± 0.25 × 107

ALV-B

293 DK-7a

1.20 ± 0.30 × 107

ALV-B/Epo

293 or 293T

293T-EpoRb

<104

9.2 ± 2.6 × 107

ALV-B/SCF

MO7-ec

8.38 ± 3.60 × 106

  1. aTransduction efficiencies of ALV-A and ALV-B pseudotypes were determined on 293 DK-7 cells, a clonal cell line expressing the chimeric TVA/TVB receptor. Vectors used were concentrated 250-fold. The titers of un-concentrated virus were 8.12 ± 4.4 × 105 (ALV-A) and 2.0 ± 0.9 × 105(ALV-B) respectively.
  2. bPseudotypes preloaded with TVA-Epo or TVB-Epo were used to transduce a 293T-based clonal cell line expressing EpoR. Vectors used were concentrated 250-fold. The titers of un-concentrated virus were 7.27 ± 0.77 × 105 (ALV-A) and 7.80 ± 0.46 × 105 (ALV-B) respectively.
  3. cPseudotypes preloaded with TVA-SCF or TVB-SCF were used to transduce c-kit-expressing MO7-e cells. Vectors used were concentrated 250-fold.
  4. dTiters presented represent the mean ± SD from three independent experiments using concentrated vector stocks.