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Figure 1 | Retrovirology

Figure 1

From: Cell-specific targeting of lentiviral vectors mediated by fusion proteins derived from Sindbis virus, vesicular stomatitis virus, or avian sarcoma/leukosis virus

Figure 1

Transduction of c-kit-expressing 293 cells using lentiviral vectors pseudotyped with the Sindbis virus strain TR339 glycoproteins and bearing SCF. (A) Schematic representation of the modified Sindbis virus strain TR339 proteins. The sequence of the modified E3 protein is shown. It encodes 66 amino acids including the signal peptide for the E2 protein. E2 consists of 423 amino acids, and the 6K and E1 proteins encode 55 and 439 amino acids, respectively. The numbers refer to the ends of the respective protein domains. (B) Representative FACS profiles. Top panels: 293-c-kit cells transduced with vectors prepared using 0 and 6 μg of the SCF-encoding pUB-HuMGF plasmid; Top left panel: Untransduced cells. Bottom panels: 293T cells transduced with vectors prepared using 0 and 6 μg of the SCF-encoding pUB-HuMGF plasmid; Bottom left panel: Untransduced cells. The percentages of EGFP-positive cells are indicated. (C) Unconcentrated vector titers on 293 cells expressing c-kit (striped bars) and on 293T cells (open bars) three days after transduction. The titers shown represent the mean ± standard deviation (SD) obtained from three independent experiments. (D) Determination of concentrated vector titers. Vectors were concentrated 300 fold by ultracentrifugation. The titers shown represent the mean ± SD from three to six independent experiments. Striped bars: 293 cells expressing c-kit; Open bars: 293T cells.

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